After losing to Plateau United on penalties in round 32 at the Samuel Ogbemudia Stadium on Wednesday, Enyimba was eliminated from the Federation Cup.
After regulation time ended 2-2, Plateau won the penalty shootout 3-2.
This time, a team from a lesser tier sent Akwa United packing as they lost to EFCC FC in a penalty shootout as well.
After the first 90 minutes concluded in a tie score, the game was decided on the spot before EFCC went on to win 9-8.
The top team in the Nigeria Professional Football League, Bendel Insurance, defeated Ahudiya 1-0 to go to the next round of the Federation Cup.
Ikorodu City FC was defeated by Kwara United 3-1, Essien Ayi by Heartland FC 2-1, and Cynosure FC by Lobi Stars 2-1.
Nigeria will compete in the 2024 CAF Confederations Cup as the tournament winner.